Identification of Genes Resistant to Bacterial Pustule Using Microarray Analysis In Soybean.

The purpose of this research was to identify genes resistant to bacterial pustule in soybean using microarray analysis, to reveal molecular mechanisms for resistance, and to apply genes for molecular breeding for soybean crop improvement.  Expression microarray slide with 42, 564 probes was constructed.  Soybean leaves from both inoculated and non-inoculated were harvesed in  3h, 12h, 1day, 3 days, and 10 days after inoculation of bacterial pustule.  From high stringency statistical analysis, we selected 1, 34, 12, 7, and 1 candidate genes from 3h, 12h, 1day, 3 days, and 10 days analyses.  From both RT-PCR and Real-time PCR, we identified 11 genes that showed same expression pattern.  In molecular function analysis of these genes from signaling pathway and metabolism,  we found out plant-pathogen interaction which may explain molecular mechanism for resistance to bacterial pustule.  Genetic linkage of these genes are discussed.  Confirmation of these genes for parents and segregatin population has been undergoing.   This results may give us information on genetic mode of each gene and the possibility of molecular breeding for resistance to bacterial pustule in soybean.