Microarray Analysis for Identification of Genes Resistant to Phytoplasma In Arabidopsis and Poplar.

Microarray analysis has been conducted to identify genes resistant phytoplasma in Arabidopsis and Poplar.  In Arabidopsis, 1,765 up-regulated and 1,432 down-regulated probes were selected out of 37,438 probes. 247, 332, 463, and 723 probes showed up-regulated expression over 5, 4, 3, and 2 times in all 3 replications, respectively. In Poplar, statistical analysis has been conducted for 1,554 probes that showed up-regulation. 90, 175, 372, and 917 probes showed up-regulated expression over 5, 4, 3, and 2 times in all 3 replications, respectively.  We selected  genes those showed same gene ontology.  These genes had 80%similarity  in gene sequence between Arabidopsis and Poplar.  From analysis of signaling pathway or/and metabolism of selected genes, we found out that these genes were involved in the signaling pathway of plant-  pathogen interaction, Phenylpropanoid pathway, and SNARE interactions in vesicular transport pathway.  Both RT-PCR and Real-time PCR have been conducting to confirm expression of these genes.