636-15 Efficiency of Marker Assisted Selection for Soybean Seed Sucrose Content.

Poster Number 319

See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Genetic Improvement of Soybean, Common Bean, and Peanut for Quality and Disease Resistance (includes Graduate Student Competition) (Posters)

Tuesday, 7 October 2008
George R. Brown Convention Center, Exhibit Hall E

Leandro Mozzoni, Univ. of Arkansas, Fayetteville, AR and Pengyin Chen, 115 Plant Science Bldg, Univ. of Arkansas, Fayetteville, AR
Abstract:
Soybean seed sucrose content ranges from 5 to 8%, and is quantitatively inherited. At least 8 genomic regions were associated with soybean sucrose seed content. Marker assisted selection for quantitative traits is desirable in order to maximize breeding efficiency; however, the markers linked to the quantitative trait loci (QTL) for sucrose content are population-specific and environment dependent. The objective of this study is, therefore, to validate marker-assisted selection for sucrose content using single sequence repeat (SSR) markers associated to previously reported QTLs. A high-sucrose parent was crossed with a conventional high-yield soybean cultivar, and the segregating population was advanced via single seed descent to the F3 generation, when lines were derived from single plants. The lines were grown in 2 environments in 2007 and 3 environments in 2008 for DNA extraction and sucrose analysis. Sucrose content was determined using anion-exchange chromatography (HPLC) coupled with an electrochemical detector. Molecular makers flanking reported QTLs were selected and confirmed to be polymorphic. Efficiency of marker-assisted selection will be assessed by contrasting the base-population mean sucrose content with the sucrose content of the individuals with and without combinations of alleles from the high-sucrose parent.

See more from this Division: C01 Crop Breeding & Genetics
See more from this Session: Genetic Improvement of Soybean, Common Bean, and Peanut for Quality and Disease Resistance (includes Graduate Student Competition) (Posters)