Antibiotics are extensively used in animal agriculture therapeutically to treat disease and subtherapeutically as growth promoters or to reduce the risk of disease outbreak in large-scale animal confinement operations.  However, a substantial amount of the antibiotics added to animal feed may be excreted in manure, which is commonly applied to agricultural land. There is growing concern that antibiotics present in manure may be available for uptake by food crops.  A six week greenhouse study was conducted to evaluate sulfamethazine uptake in corn, lettuce, and potato from manure-amended soil.  Three treatments were evaluated: control (no antibiotic addition), 50 mg sulfamethazine L^-1 manure, and 100 mg sulfamethazine L^-1 manure. Antibiotic analysis was conducted using a combination of enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) technologies. The results showed plant uptake of sulfamethazine by all three crops. The crop uptake followed the trend lettuce > corn > potato. Sulfamethazine concentration in plant tissue increased with an increase in its concentration in manure. Maximum plant uptake corresponded to 1.1 mg kg^-1 (dry weight) for lettuce. This research points to concerns of antibiotics appearing in food chain from manure applied soils. This may have some implications in organic food production where manure is a main source of nutrients for plants.