Antioxidants are compounds in foods that inactivate harmful compounds called free radicals which can damage cells and cause diseases such as cancer. In addition to health benefits, antioxidants can reduce rancidity in foods, and there is evidence that antioxidants have a role in reducing biotic and abiotic stresses in plants Oat (Avena sativa L.have several antioxidants. Among the more abundant are Vitamin E-like compounds alpha-tocopherol (ATP) and alpha-tocotrienol (AT3), collectively called “tocols”. Considering the benefits of antioxidants, increasing the concentration of the compounds in oats would be a worthy objective for oat breeders, but no genetic information on oat tocols is available to breeders. The objective of this study was to use an oat mapping population to get an indication of how many genes (quantitative trait loci, QTL) contribute to tocol expression and which mapped markers the tocol QTL are linked to. To achieve this, an ‘Ogle' x ‘TAMO-301' (OxT) recombinant inbred mapping population, parents, and check cultivars ‘Monida' and ‘Ajay' were grown in 0.122 x 0.244-mfour-row plots in six location x year environments in two blocks per environment. Dry seed from each plot were ground to flour and tocols were extracted induplicate with an organic solvent. Samples of each extraction were fractionated with HPLC and detected with fluorescence and reported as μg tocol/g flour. Data from duplicates and blocks were averaged and the entry means were analyzed with the NQTLprogram. For ATP, simple interval analysis inferred three significant QTL on OT linkage groups 9, 16, and 30. Two QTL were inferred for AT3 on OT linkage groups 15 and 33. Of the check cultivars, Ajay tended to have elevated tocol levels, especially AT3. This information will lead to the development of molecular markers and potential crosses to guide breeders toward enhancing tocols in oat.