Genetic engendering is of significant interest to improve crops productivity and therefore contribute to grower's profiatabilty and /or add value to certain traits that are of interest to users. Recent progress in transformation methods has allowed the transfer and expresses of many genes such as herbicide resistance from other species into wheat. Sixteen genotypes with Glyphoste (Roundup) resistance gene (event 33391) developed by North Dakota State University, South Dakota StateUniversity, and the University of MN in collaboration with Monsanto were included in a study installed in 3 the states (ND, SD, and MN) from 2001-2004. The study aimed to examine the agronomic performance and quality parameters of roundup transgenic wheats under filed condition in the Northern plains of the USA. Data from more than 20 year-locations showed the performance (Yield, yield components and other agronomic traits) of the elite wheat lines with roundup ready gene was variable suggesting that the gene does not affect any of the studied traits. Similarly more than 22 quality (milling and baking) traits, showed significant variations among all genotypes regardless of presence or not of the roundup resistance gene. Many lines with the round up genes have outperformed the widely grown checks in three states in term of yield and /or quality .