In Vitro Propagation of Native Bigtooth Maple (Acer grandidentatum).
Poster Number 1305
Monday, November 4, 2013
Tampa Convention Center, East Hall, Third Floor
Kyle Bair and Daniel Dewey, Brigham Young University-Idaho, Rexburg, ID
Tissue culture has not been used to propagate mature tissue of native bigtooth maple (Acer grandidentatum). The purpose of this experiment was to develop a protocol to root hardwood cuttings by tissue culture.Hardwood cuttings were acquired from nursery grown trees at Utah State University. The cuttings were divided into four treatments: 10 µM indole-3- acetic acid (IAA), 10 µM l –naphthaleneacetic acid (NAA), 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D), and 10 µM indole-3-butyric acid (IBA). Each cutting was randomly chosen from different sections of the stem. Furthermore each cutting contained one and/or two nodes per explant. Shoot proliferation occurred with the explants exposed to IAA, NAA, and 2,4-D. After a period of 27 days, several successfully budded explants were moved to a non-growth hormone media for later comparison with those cuttings which remained in growth hormone.