The aim of this research was to verify the influence of Macrophomina phaseolina, Colletotrichum dematium f. truncata and Colletotrichum lindemuthianum in the physiological quality of bean seeds. There were used seeds from Carioca and FT Nobre cultivars. The seeds were artificially inoculated with fungi M. phaseolina, C. dematium f. truncata and C. lindemuthianum in BDA medium culture with and without hydric restriction it was added -1,0 MPa manitol. Seeds were placed on the medium culture for 16 hours for M. phaseolina and 48 hours for C. dematium f. truncata and C. lindemuthianum. Seeds were evaluated, by the blotter test, sand germination and vigor tests. Analyses from the imbibition water of the seeds in the electrical conductivity test, were also done, as well as in the medium culture. Infection of bean seeds with M. phaseolina for the period of 16 hours and C. dematium f. truncata and C. lindemuthianum for 48 hours with the use of manitol - 1,0 MPa for hydric restriction is enough to verify the interference of these organisms in the physiological quality of the seeds. The germination bean seeds for both cultivars is affected by M. phaseolina and not for C. dematium f. truncata and C. lindemuthianum; in the vigor tests like a accelerated aging, cold and electrical conductivity of FT Nobre cultivar is more affected by thefungi; infected seeds with M. phaseolina and C. lindemuthianum present lower values of electrical conductivity than in healthy ones.