Water availability is critical to plant productivity in water-limited environments. Irrigation management and cultivar selection can enhance yield and quality under water limited conditions. Boll distribution is an important parameter of yield in cotton, and very sensitive to a variety of stresses and environmental conditions. The changes in boll distribution pattern are frequently used to quantify the effects of biotic and abiotic stresses on cotton yield and quality. So, cotton prices are affected by both yield and quality. Due to indeterminate growth pattern of cotton, the fiber properties will vary within each mainstem node, as they develop at different times during the growing season. The main objectives of this research were to study the boll distribution interaction with cultivar and irrigation and study the variation in fiber properties due to irrigation and variety within each main-stem nodes.