Monday, June 18, 2007
Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis.
Michael S. Gomez1, Nicholas Denwar1, T. Ramasubramanian2, Gloria Burow3, John Burke3, Naveen Puppala4, and Mark Burow5. (1) Department of Plant and Soil Science, Texas Tech University, Texas Tech University, Box 42122, Lubbock, 79409, (2) Department of Biotechnology, Tamilnadu Agricultural University, Coimbatore, 641003, India, (3) 3810 4th St, USDA-ARS, USDA-ARS, Cropping Sys Res Lab, Lubbock, TX 79415, (4) New Mexico State University, New Mexico State University/ASC at Clovis, 2346 Sr 288, Clovis, NM 88101, (5) Agricultural Experiment Station, Texas A&M Univ., 1102 E. FM1294, Lubbock, TX 79403
Microsatellite markers are a tool for identification of true F1 hybrids and for assessing genetic purity. Peanut has a compound inflorescence comprised of three flowers appearing at the same axil over the course of approximately one week. This allows for accidental misidentification of crosses and inadvertent selfs. For this reason, SSR markers would be useful for classifying intended hybrids. We have developed a system for this using a simplified horizontal polyacrylamide gel electrophoresis technique. Polyacrylamide gels were run in a submarine horizontal electrophoresis apparatus designed for agarose gels. The electrophoretic mobility of microsatellite fragments obtained in PAGE gels gave better band separation and differentiation of selfed progenies from true peanut hybrids better than use of SFR agarose. This method is less expensive than SFR agarose and amplified bands are very clear and sharper than SFR agarose gels.