Large-scale field trials were conducted at the University of Wyoming Torrington Research and Extension Center in 2003 and 2004 to evaluate the effect of row spacing on two sugarbeet cultivars. Experimental design was a split-plot randomized complete block with row spacing as the whole-plot factor and sugarbeet cultivar as the split-plot factor. Sprinkler irrigated plots were established in 2003 and 2004 and were 10 by 600 feet with four replications. Furrow irrigated plots were established in 2004 only and were 10 by 300 feet with three replications. Sugarbeet cultivars ‘Beta 4546’ and ‘Crystal 9906’ were planted into 15- or 30-inch rows on April 15 in both years at a rate of 70,000 seeds per acre. Due to dry conditions and inability to irrigate for emergence, the furrow irrigated trial in 2004 was replanted on May 27. Row spacing had no effect on sugarbeet stand. A row-spacing by cultivar interaction was observed with respect to sugarbeet root yield. Beta 4546 yielded over 5 tons per acre more in 15-inch rows compared to 30-inch rows, while Crystal 9906 yielded over 3 tons per acre more in 15-inch rows than in 30-inch rows.  No significant differences were observed with respect to root yield between cultivars in 15-inch rows, however, Crystal 9906 yielded over 2 tons per acre more than Beta 4546 when planted into 30-inch rows. Sucrose content and sugar loss to molasses were not affected by row spacing.