Root-knot disease caused by Meloidogyne incognita is a matter of grave concern because it affects several economically important crop plants. Apply of the gelatinolytic bacteria in reducing the reproduction rate of root-knot nematodes in tomato is regarded as one of the new attempts. Three gelationlytic bacteria were isolated from rhizosphere soil that had been enriched with gelatin. These bacteria were identified as Acinetobacter lwoffii, Bacillus luciferensis and Bacillus anthracis based on 16s rRNA gene sequencing. Cultures of the bacteria were found to destruct the female of M. incognita under microscopy investigation in vitro. At two weeks after transplanting, each 10 ml culture of Acinetobacter Lwoffii (AL), Bacillus Luciferensis (BL) and Bacillus Anthracis (BA) grown in a medium containing gelatin (10 g/L) as sole carbon source was added into tomato rhizosphere to investigate the efficacy in biological control against Meloidogyne incognita. Control (CO) received same quantity of water. At 3 weeks, 10 eggmasses picked up from the infested roots were inoculated to each pot and then samples were taken at 4, 5 and 6 weeks after transplanting. Shoot length and fresh weight showed higher values, while root fresh weight at AL, BL and BA treatments had lower values compared to CO treatments. Population of the gelatinolytic bacteria was greater in the soils amended with gelatinolytic bacteria. Second stage juveniles, eggmasses and gall index were lower in AL, BL and BA compared to CO treatment at each time point. Populations of the gelatinolytic bacteria in the tomato rezosphere were negatively correlated with the second stage juveniles, eggmasses and gall index. These results indicate the potential of the gelatinolytic bacteria to alleviate nematode parasitism in important vegetable crops.