Trinitrotoluene (TNT) and its derivatives 2,4-DNT and 2,6- DNT are toxic organic compounds widespread in soil and water environments due to runoff, industrial wastes sites, and military testing sites. Thus, these compounds are significant environmental pollutants of considerable concern. Biodegradation and photolysis have been reported as the major mechanisms of environmental fate and transport of these compounds. This field study was, therefore, conducted to investigate these two compounds in self-contained undisturbed soil lysimeters on the Eastern Shore of Maryland in 2004. The experiment was conducted on an Othello soil, and arranged in a randomized complete block design with eight treatments and three replications at depths of 30 and 60 cm. Soils were thoroughly mixed with 0, 25 and 50 mg/kg soil of 2,4-DNT and 2,6-DNT. Soil core samples were collected at 0-10, 10-20, 20-30, 30-40 and 40-60 cm after 0, 30, 60, 120 and 180 days. These cores were examined for nitrate-N, nitrite-N, pH, 2,4-DNT, 2,6-DNT, and their metabolites using a GLC-FID Model-6890. A laboratory experiment was also conducted to examine the role of rhizobia and bradyrhizobia on the bioremediation of these environmental pollutants. Results indicate that hydrogen ion concentration values varied slightly among treatments over time, and ranged from 6.1-7.8. Nitrate-N continuously increased over time while nitrite-N was undetectable. The increase in the amount of nitrate-N varied among treatments and with soil depth. The amounts of 2,4-DNT and 2,6-DNT were the highest in the top 0-10 cm of the soil throughout the experiment, and decreased with depth over time. Six metabolites of the above organic compounds were found. The metabolism of these chemicals by rhizobia and bradyrhizobia depended on the bacterial strains applied. This study provides pertinent information relative to the fate and transport of these hazards compounds in soil environments.