Friday, 14 July 2006
104-6
Detection of Salt Tolerance Using Chlorophyll Fluorescence Photometer.
Sei Joon Park1, Ju Young Lee2, Sang Eun Lee3, Sung Yung Yoo3, Myoung Yong Shim1, Seung Gil Yun3, and Tae Wan Kim3. (1) Institute of Ecological Phytochemistry, Hankyong National University, 67 Seogjung-dong, Anseong, 456-749, South Korea, (2) Division of Plant Nutrition, National Institute of Agricultural Science and Technology, RDA, 250 Seodun-dong, Suwon, 441-707, South Korea, (3) Department of Plant Resources Science, Hankyong National University, 67 Seogjung-dong, Anseong, 456-749, South Korea
Sugar beet seeds were directly sawn and grown in salt treated soils. Soil electronic conductivity (EC) was controlled to 2, 4, 7, 9 and 12 dSm-1 with NaCl. Photosynthetic activity was measured in 30 day old plants using chlorophyll fluorescence photometer. After a 30-min dark period in ambient conditions in the laboratory, chlorophyll a fluorescence in soybean leaf was measured using a pulse-amplitude modulated fluorometer (Fluorcam, CZ). Measurements of minimal (F0) and maximal (Fm) fluorescence yields allowed determination of the optimal quantum yield (Fv/Fm), the ratio (Fm – F0)/Fm being used to calculate the maximal potential efficiency of PS II of dark adapted leaves. Fv/Fm was reduced over 9 dSm-1 EC. Non-photochemical quenching (NPQ) was highest in leaf grown over 9 dSm-1 EC soil. PSII efficiency factor(F'q/F'v) was also considerably lowered in leaf grown over 7 dSm-1 EC soil. Key words: chlorophyll fluorescence, non-photochemical quenching, screening, salt stress. Acknowledgment: This study was financially supported from Bio-Green 21 project, RDA and in part from GRRC project (No. 3-1), Hankyong National University. Corresponding author: Tae Wan Kim, taewkim@hknu.ac.kr
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