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Tuesday, November 3, 2009
Convention Center, Exhibit Hall BC, Second Floor
Abstract:
Barley S-adenosylmethionine synthetase (HvSAMS) catalyses the biosynthesis of S-adenosylmethionine (SAM) from ATP and L-methionine. HvSAMS 1, 2, 3, and 4 genes and their 5' upstream regions were isolated and analyzed.
The genomic DNA sequences of HvSAMS 1, 2, 3, and 4 genes did not contain intron. However, two introns and two exons were present on the 5' upstream regions of each HvSAMS 1, 2, 3, and 4. They were present within 1787 bp (HvSAMS 1), 1698 bp (HvSAMS 2), 1916 bp (HvSAMS 3), and 1537 bp (HvSAMS 4) from the translated initiation site.
Although high sequence homology was found among the four HvSAMS genes and first exon on the 5' upstream region, numerous deletions and insertions were found in the second exon from the 5' upstream region and two introns. The second exon from the 5' upstream region among HvSAMS 1, 2, 3, and 4 showed high structural variation.
In order to identify proposed alternative splicing occurred in 5' upstream region, RT-PCR with exons and introns of promoter specific primers were conducted using barley grains of different developmental stages. Presence of different transcript sequences derived from the 5' upstream region might indicate different upstream sequence use of HvSAMS genes.
Acknowledgement
This work was supported by a grant from the BioGreen 21 Program (20070301034016), Rural Development Administration, Republic of Korea, and partially supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD: KRF-2007-357-F00001)
The genomic DNA sequences of HvSAMS 1, 2, 3, and 4 genes did not contain intron. However, two introns and two exons were present on the 5' upstream regions of each HvSAMS 1, 2, 3, and 4. They were present within 1787 bp (HvSAMS 1), 1698 bp (HvSAMS 2), 1916 bp (HvSAMS 3), and 1537 bp (HvSAMS 4) from the translated initiation site.
Although high sequence homology was found among the four HvSAMS genes and first exon on the 5' upstream region, numerous deletions and insertions were found in the second exon from the 5' upstream region and two introns. The second exon from the 5' upstream region among HvSAMS 1, 2, 3, and 4 showed high structural variation.
In order to identify proposed alternative splicing occurred in 5' upstream region, RT-PCR with exons and introns of promoter specific primers were conducted using barley grains of different developmental stages. Presence of different transcript sequences derived from the 5' upstream region might indicate different upstream sequence use of HvSAMS genes.
Acknowledgement
This work was supported by a grant from the BioGreen 21 Program (20070301034016), Rural Development Administration, Republic of Korea, and partially supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD: KRF-2007-357-F00001)