/AnMtgsAbsts2009.54707 Screening Effective Co-Inoculant Bacterial Strains Containing ACC-Deaminase Activity for Promoting Growth and Nodulation of Chickpea Seedlings Under Axenic Conditions.

Wednesday, November 4, 2009: 10:15 AM
Convention Center, Room 306, Third Floor

Sher Shehzad1, AZEEM KHALID2, Muhammad Arshad3, Zahir Ahmed Zahir4, Rizwana Kausar4, Muhammad Saleem Arif4 and Muhammad Anjum4, (1)UNIVERSITY OF AGRICULTURE, FAISALABAD, Inst. of Soil & Environmental Sciences, Faisalabad, Pakistan
(2)Dep. of Environmental Sciences, Rawalpindi, Pakistan
(3)Institutue of Soil and Environmental Sciences,, Univ. of Agriculture, Faisalabad, Faisalabad, Pakistan
(4)Inst. of Soil & Environmental Sciences, Faisalabad, Pakistan
Abstract:
A multi-dimensional beneficial association between rhizobia and leguminous plants has been observed which consequences in the development of nodules on the roots of legumes. Besides various other features, plant hormones have an imperative function for increasing nodulation in legumes. Certain free living rhizobacteria promote nodulation in plants by regulating endogenous ethylene biosynthesis in roots due to activity of ACC-deaminase enzyme. Six pre-selected isolates of each rhizobia and rhizobacteria were evaluated in all possible combinations for prompting growth and nodulation of chickpea by conducting growth pouch and jar experiments. The results of pouch and jar experiments showed that co-inoculation of chickpea with rhizobia and rhizobacteria containing ACC-deaminase resulted in significant increase in root and shoot growth as compared to uninoculated control. Some of the co-inoculation combinations were very effective in producing high number of nodules as well as dry nodule weight as compared to uninoculated control. The findings suggest that co-inoculation with PGPR containing ACC-deaminase and rhizobia could be useful for improving the growth and yield nodulation of chickpea. The study also facilitates us to select effective combinations for co-inoculation to promote nodulation and yield of chickpea under natural conditions.

Mode of Presentation: Oral

Correspondence: Sher Muhammad Shahzad

Ph.D. Students, Institute of Soil & Environmental Sciences, University of Agriculture, Faisalabad, Pakistan