Suman Andru and Collins A. Kimbeng. Louisiana State University, 104 MB Sturgis Hall, Department of Agronomy and Environmental Management, Baton Rouge, LA 70803
Modern sugarcane cultivars were derived from interspecific hybridization between two major Saccharum species, namely S. officinarum and S. spontaneum. The objective of the study was to assess the molecular diversity in a local collection of S. spontaneum and S. officinarum clones and to survey the proportion of this diversity represented in major Louisiana breeding clones using TRAP and SRAP markers. Among a total of 61 clones, 40 were Saccharum spontaneum, 10 were S. officinarum and 11 were major Louisiana commercial breeding clones. A total of 12 TRAP and 12 SRAP primer combinations was used to genotype the clones. All the primers amplified a large number of markers due to the polyploid nature and high level of heterozygosity of the sugarcane genome. Cluster analysis and PCA will be used to study the relationships between the three groups of entries. Structure analysis will be used to detect the proportional representation of ancestral genomes in each cultivar. The results will be useful in identifying and utilizing ancestral clones that are least represented in the commercial breeding gene pool.
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