Podophylotoxin is an anti-cancer compound found in high concentration in American mayapple (Podophyllum peltatum).  In recent years, a significant amount of research has been conducted at Mississippi State University and the University of Mississippi on American mayapple. The objective of this work was to continue with the bioprospecting of American mayapple in order to identify accessions with high podophylotoxin content. Thirty-eight mayapple colonies have been sampled across Mississippi, Alabama, Arkansas, Georgia, Kentucky, Louisiana, Maryland, Massachusetts, New York, New Hampshire, North Carolina, Ohio, Pennsylvania, South Carolina, Tennessee, Texas, Virginia, and West Virginia.  Overall, the colony yields and podophylotoxin content varied among locations and accessions.  The results from this study will help to develop a GIS (Geographic Information System) map of the genetic resources of American mayapple in the US.