See more from this Session: Use of Molecular Tools to Enhance Breeding Efforts
Tuesday, November 2, 2010
Long Beach Convention Center, Exhibit Hall BC, Lower Level
Random amplified polymorphic DNA (RAPD) is widely used technique for genetic fingerprinting, but the practical applications of such markers are enhanced by sequencing the RAPD products and converting them more specific SCAR (sequence-characterized amplified region) markers. The objective of the present study was to generate SCARs marker for identification of Perilla frutescens cultivars. Also, this study could offer the derivation of more reliable PCR markers. Seven of the RAPD markers were wholly sequenced and new sequence-specific primers were synthesized for use under more stringent amplification conditions. The positive identification SCAR markers for 30 Perilla frutescens cultivars were obtained 7 specific primer pairs.