Comparative Analysis of Buffalograss [Buchole Dactyloides (Nutt.) Engelm], Blue Grama (Bouteloua gracilis [H.B.K]. ex Steud.) and False Grama [Cathestecum Erectum Vasey & Hack].
Tuesday, November 3, 2009
Convention Center, Exhibit Hall BC, Second Floor
Bekele G. Abeyo1, Robert C. Shearman2, Desalegn Serba3, D.M. Kopec4 and Donald J. Lee2, (1)Dept. of Agronomy and Horticuture, Univ. of Nebraska, Lincoln, Lincoln, NE (2)Agronomy and Horticulture, Univ. of Nebraska, Lincoln, Lincoln, NE (3)Univ. of Nebraska, Lincoln, Lincoln, NE (4)Tucson, AZ, United States of America
Buffalograss and blue grama
dominated the short grass prairies of the Great Plains of North America, while
false grama is found mostly in the semiarid regions
of Texas and Arizona. Information about their genetic
diversity or similarity is limited, and more research is needed to better
understand genetic diversity. This study was initiated to assess the
level of genetic diversity within and among buffalograss,
blue grama, and false grama
genotypes, using morphological and molecular markers. Twelve genotypes of
each species were evaluated using simple sequence repeat (SSR) markers derived
from pearl millet. In addition, some morphological traits were measured.
Buffalograss and false grama are stoloniferous,
while blue grama is a bunch-type grass. Buffalograss
is mostly dioecious, while blue grama
and false grama are monoecious.
The three species slightly differ in their flower location, color and
morphology. Buffalograss has shorter internodes than the other two, while blue grama and buffalograss have significantly
lower number of internodes than false grama. Most
markers used were amplified in buffalograss with
polymorphic and monomorphic bands, but amplification
and polymorphism were limited in blue grama,
indicating that selections with similar genetic background were used.
Cluster analysis based on 19 SSR markers showed that genotypes of each
species were distinctly clustered with higher variability occurring in buffalograss, false grama being
intermediate and blue grama being least in
variability. Based on these results all genotypes of buffalograss
and blue grama were uniquely separated and distantly
related with each other, false grama fell between the