Monday, June 22, 2009: 3:05 PM
Room 214-216 Lory Student Center, CSU
Bread making quality is critically important for U.S. hard winter wheat (Triticum aestivum). This study was conducted to identify quantitative trait loci (QTLs) that influence quality traits in a doubled haploid (DH) population derived from the cross of ‘Platte’ and CO940610. Field trials of 186 DH lines were grown in Fort Collins, CO in 2008 under moisture stress and fully irrigated conditions. Quality traits were evaluated with a 10-g Mixograph, single kernel hardness tester, and near infrared spectroscopy. We evaluated 186 simple sequence repeat (SSR) and diversity array technology (DArT) markers in each DH line, and constructed preliminary maps of 28 linkage groups with Join Map 4 software. The composite interval mapping option of QTL Cartographer software was used in a genome-wide scan for significant QTLs. The population showed broad ranges of trait values, with transgressive segregation apparent for most traits. We detected 59 QTLs for end-use quality traits, distributed on most of the linkage groups. Genomic regions on chromosomes 1A, 1D, and 7B contained QTLs for multiple traits, with most of these QTLs detected under both moisture stress and fully irrigated conditions. The 1A and 1D QTLs most likely indicate the effects of the Glu-A1 and Glu-D1 loci, whereas the 7B QTL region may reflect a novel quality locus or loci. We will repeat the QTL analysis with data from the 2009 trials of this population. If the QTLs are validated, they may be useful for marker assisted selection or high resolution mapping leading to map-based cloning.
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