Chromosome Doubling of Haploid Maize Seedlings by Various In Vitro Treatments.

A two-year study was conducted on methods for doubling haploid maize plants with elite temperate backgrounds following various seed treatments.  Seedlings were treated with colchicine or other chemicals by 1) injecting plants and 2) a soak method using various seedling treatments.  Treated seedlings were transplanted in the field in 2010 and 2011, and doubled plants that shed pollen and produced ear shoots were pollinated to derive double haploid inbred lines.  Results and conclusions will be reported for efficacy of doubling, and other morphological traits observed in the field.