Methodology for Rapid Differentiation of Genotypes of Cotton (Gossypium Spp.) with Molecular Markers.

There is a wide range of genotypes of cotton in the world and only a few gene banks that collect all genotypes. There are no methodologies to differentiate genotypes. Thus, it was necessary to develop such tools in order to facilitate the rapid identification of genotypes for cotton breeding. We developed a molecular key from specific molecular primers for the differentiation of alloplasms of cotton (Gossypium Spp.). The key was developed from specific molecular PCR markers that were unique to a species.  This allowed differentiation of genotypes of cotton based on mitochondrial and chloroplast DNA  The genomic group and species used for  this research were A1 (G. herbaceum), B1 (G. anomalum), B3 (G. capitis-veridis), C1 (G. sturtianum), D1 (thuberi), D2-1 (G. armorianum), D3-d (G. davidsonii), D4 (G. aridum), D5 (G. raimondii), D8 (G. trilobum), E2 (somalense), F (longicalyx) and G (australe). The evaluation of the different genotypes of cotton with each marker, revealed a unique band for each species. Thus we were able to obtain a marker for each species and to develop the key that distinguished eleven species of cotton. However, at the time this abstract was prepared a unique fragement that distinguished the genome A from the G genome had not been observed.  The latest version of the key will be presented.