Proteomic Analysis of Primed Canola Seeds.

Seed germination is a complex phenomenon, as population of seeds does not perform this process uniformly. It start with imbibition i.e. uptake of water by seed and ends when radical emerges out of the seed through seed coat. But there is no universal biochemical markers that can determine the progress of germination that can help the seed industry in determine the seed quality and its improvement using the priming methods. Therefore this study was planned to determine the changes in protein profile of canola seeds as the process of germination progresses. Canola seeds were soaked in water and protein was extracted from dry seeds, and 24hrs and 48hrs after soaking. Then proteins were analyzed by one dimension and two dimension SDS PAGE gel electrophoresis. Results of  1-D SDS PAGE gel electrophoresis showed that as the germination progresses the proteins with molecular weigh ~60kD over expresses linearly from dry seeds to 24hrs soaking period and then 48 hrs soaking period. Same expression pattern was observed in 2-D gel electrophoresis.