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Poster Number 377
See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
See more from this Session: Genetics of Abiotic and Biotic Resistance (Posters)
Wednesday, 8 October 2008
George R. Brown Convention Center, Exhibit Hall E
Abstract:
ABSTRACT
Downy mildew of sunflower caused by the obligate parasite Plasmopara halstedii is one of the major diseases of cultivated sunflower (Helianthus annuus L.). Resistance to downy mildew has been found for all the races of P. halstedii. At least 11 resistance genes, designated as Pl, were reported to provide resistance to one or more races. Bulked-segregant analysis was conducted with SSR markers on 208 individual F2 plants from a cross between a downy mildew susceptible line H251 and resistant line OND163R. Six SSR markers closely linked to downy mildew resistance were identified and mapped on linkage group 8. The marker HA1504 co-segregated with the resistance and the additional five markers (HA0100, HA0438, HA0487, HA0505 and HA1291) were located at 1 cM from the resistance locus. The fact that the resistance line OND163R was derived from HA337, which was reported to contain Pl7, suggested that the SSR markers we identified might be linked to Pl7. These SSR markers were further validated on two F2 populations of different genetic backgrounds and have been utilized in a Marker Assisted Selection program for the resistance to downy mildew in sunflower.
Downy mildew of sunflower caused by the obligate parasite Plasmopara halstedii is one of the major diseases of cultivated sunflower (Helianthus annuus L.). Resistance to downy mildew has been found for all the races of P. halstedii. At least 11 resistance genes, designated as Pl, were reported to provide resistance to one or more races. Bulked-segregant analysis was conducted with SSR markers on 208 individual F2 plants from a cross between a downy mildew susceptible line H251 and resistant line OND163R. Six SSR markers closely linked to downy mildew resistance were identified and mapped on linkage group 8. The marker HA1504 co-segregated with the resistance and the additional five markers (HA0100, HA0438, HA0487, HA0505 and HA1291) were located at 1 cM from the resistance locus. The fact that the resistance line OND163R was derived from HA337, which was reported to contain Pl7, suggested that the SSR markers we identified might be linked to Pl7. These SSR markers were further validated on two F2 populations of different genetic backgrounds and have been utilized in a Marker Assisted Selection program for the resistance to downy mildew in sunflower.
Key words: Sunflower, downy mildew, SSR markers
See more from this Division: C07 Genomics, Molecular Genetics & Biotechnology
See more from this Session: Genetics of Abiotic and Biotic Resistance (Posters)