Development and Application of High Throughput Process for Mapping Putative Transgene Insertion Sites.

The ability to rapidly and accurately identify putative insertion sites for selected transgenic events is a pre-requisite for marker assisted introgression.   The objective was to develop a reproducible process to efficiently map transgene locations while balancing key concepts of mapping, commercial timelines, and resources.  To significantly reduce genotyping costs, we employed bulk segregant analysis followed by selective genotyping.   The resulting automated processes enable researchers to efficiently characterize multiple transgenic events.  Described is the development and application of a high throughput process for identification of transgene insertion sites, with success measured by observations in segregating progeny.