An F2:3 population derived from a cross between a FHB susceptible parent (KY93C-1238-17-2) and a FHB resistant parent (VA01W-476) was divided into two subpopulations. The first subpopulation was subjected to genotypic selection. Thus, 10 seeds from each of 48 F2:3 heads were planted in pots in the greenhouse in 2006. Each plant was evaluated for the presence of the major 3BS QTL associated with FHB resistance using three markers (barc147, gwm533 and Fhb1). Seeds coming from plants homozygous resistant and susceptible for the 3BS QTL were planted in headrows in 2007 to form two contrasting genotypically selected populations. The second F2:3 subpopulation comprised 48 heads planted in headrows in 2006; the objective was to subject this population to phenotypic selection. Seed from the phenotypically selected subpopulation formed the third population planted in headrows in 2007. In 2008, disease ratings will be recorded in the field ; percentage of Fusarium damaged kernels (FDK) and deoxynivalenol (DON) content will be measured in seed harvested from the headrows. The three populations will be compared for genetic progress in scab resistance. The results of this experiment will help to determine the optimal balance for genotypic and phenotypic selection in FHB resistance selection programs.
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