Nitrogen is an essential nutrient for cotton, and N deficiency affects crop growth, fruiting, lint yield and quality. This study was conducted to determine the relationships between leaf nitrogen, cotton growth, and lint yield of four transgenic cotton cultivars (DP 555B/R, FM 960B/R, SG 215BG/RR and ST 559B/R) across four N fertilizer rates on a Leeper silt clay loam at Mississippi State (33°28’N, 88°47’W), MS. The treatments were: (1) no N applied during the growing season; (2) 56 kg N ha^-1 applied at the second true leaf stage; (3) 112 kg N ha^-1, equally split (56 and 56 kg ha^-1) and applied at the second true leaf stage and at the first square stage; and (4) 168 kg N ha^-1 split into two applications of 56 and 112 kg N ha^-1 and applied at the second true leaf stage and at the first square stage, respectively. Plant height, mainstem node number, leaf area and above-ground plant component dry weights and N were measured at specific growth stages. Dates of squaring, flowering and boll opening were also recoded. At the end of the season, lint yield and fiber quality were also measured. In addition, 1-m row of plants were mapped to understand cultivar X N interaction on boll retention and weight distribution patterns. Nitrogen application did not affect squaring, flowering and boll opening dates while cultivars differed only by 2-3 days for those events. No significant interactions were observed between N and cultivars. Averaged over years and cultivars, lint yield increased with applied N rates, 911, 1000, 1207 and 1341 lb/acre for the 0, 56, 112 and 168 kg N, respectively. Nitrogen treatments and cultivars differed on boll distribution patterns and lint quality parameters.