Two field experiments were conducted in 2006 at five locations in Mississippi to evaluate sunflowers as oilseed crop for biodiesel production.  The first experiment evaluated the effect of location, variety, and N rate application, while the second experiment evaluated the effect of location, variety, and seeding date.  Four and nine conventional sunflower varieties were used in the first and the second experiment, respectively.  The N rates in the first experiment were 0, 60, 120, and 180 kg/ha.  The seeding dates were in April, May, and June for both experiments.  The first experiment was conducted in Newton, Stoneville, Starkville, and two locations in Verona, MS.  The second experiment was conducted in Beaumont, Newton, Starkville, and two locations in Verona.  Overall, the first seeding date was successful and resulted in relatively good seed yields from all sunflower varieties in all locations.  Due to the drought conditions in early summer of 2006, the second and the third seeding date did not establish in all locations, and the yields were low.  Potential production issues with sunflower production in Mississippi seem to be the control of black birds, some insects such as stem borers, and control of nutsedge, morning glory, and some grasses.  Our one-year multilocation data suggest that sunflowers could be a viable oil seed crop for biodiesel production in most parts of Mississippi.