Molecular Fingerprinting and Association with Phenotyping in Near Isogenic Introgression Lines of soybean (Glycine max).
Wen-Di E1, Guo-An Zhou2, Rong-Xia Guan2, Ru-Zhen Chang2, and Li-Juan Qiu2. (1) Northeast Agricultural University, Harbin, 150030, China, (2) The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Lab of Germplasm & Biotechnology, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Near-isogenic ingression lines (NIILs) are useful tools for precise mapping of quantitative trait loci (QTLs) and the evaluation of gene action or interaction. A set of 112 NIILs carrying variant introgressed segments in the same background, was screened by using 179 polymorphic SSR loci. The 112 NIILs represented 70.67% of the genome of donor parents. All NIILs had the proportion of the recurrent parent ranging from 90.38% to 99.53%, with the average of 97.30%. A total of 631 donor segments were detected, including 427 homozygous and 204 heterozygous segments?with each NIIL containing 3-5 segments. The introgressed segments length was ranged from 0.14 to 58.73 cM, with the average of 11.55 cM. The number and coverage of the introgressed segments were different among different linkage groups. Associated analysis showed that a total of 24 SSR loci were associated with agronomic traits at significant or extremely significant level, of which 1 locus was related to maturity date (R2>25%), 10 loci were associated with the seed weight (R2>30%), 6 loci were associated with the height, 3 loci were associated with the crude protein content and 4 loci associated with oil content. These NIILs will be useful in identifying important genes related with some favorable traits.