Methods for Developing a Greenhouse Screen to Determine Sting Nematode Response in Zoysiagrass Germplasm.
Brian Schwartz1, Kevin Kenworthy2, William Crow3, Kenneth Quesenberry2, David Wofford2, and Grady Miller4. (1) University of Florida, 304 Newell Hall PO Box 110500, Gainesville, FL 32611-0500, (2) Agronomy, University of Florida, 304 Newell Hall PO Box 110500, Gainesville, FL 32611-0500, (3) Entomology and Nematology, University of Florida, PO Box 110620, Gainesville, FL 32611-0620, (4) North Carolina State University, NCSU Department of Crop Sciences, Campus Box 7620, Raleigh, NC 27695
Widespread infestation of the plant-parasitic sting nematode, Belonolaimus longicaudatus, has been described throughout Florida and on Zoysia japonica as early as the 1950's. Sandy, well-drained soils in Florida provide a suitable environment for this ectoparasite to reproduce and maintain large population numbers which are able to inflict significant injury on turfgrass. A perceived susceptibility of zoysiagrass to the sting nematode currently limits its use on golf courses and home lawns constructed primarily on sand-based soils. Therefore, an experiment was conducted to quantify the level of sting nematode resistance/tolerance of four zoysiagrass cultivars in comparison to negative (St. Augustinegrass) and positive (bermudagrass) check cultivars. A factorial design was employed to discern the main effects and interactions of three potting methods, each inoculated at two levels and replicated six times. Resistance was based on the number of nematodes present 90 days after inoculation. Tolerance levels were identified by comparing root lengths of inoculated plants with non-inoculated plants.