Sudden death syndrome (SDS) of soybean, caused by Fusarium virguliforme, is a recurrent disease in the soybean growing regions of the United States and South America. The objective of this research is to study the inheritance of resistance to SDS in Hartwig and identify the microsatellite markers linked to SDS resistance. A population of 150 F_2:3 lines derived from Camp x Hartwig were inoculated and screened in the greenhouse for their SDS reactions. The results showed at least one gene for SDS resistance was postulated on linkage group MLG in Hartwig which was inherited from its parent Forrest. Nine single sequence repeats (SSRs) primers on MLG were identified to be associated with this gene. This research can provide breeders for marker-assisted selection of SDS resistance.