Wednesday, November 15, 2006
238-3
Pesticide Degradation by Trametes versicolor in Organic Matrixes under Laboratory Conditions.
Graciela Ruiz-aguilar, Universidad de Guanajuato, UNESS, "Privada De Arteaga S/n, Centro", Salvatierra, GTO 38900, MEXICO, Enoc Rico-Jordan, "Universidad de Guanajuato, UNESS", "Privada De Arteaga S/n, Centro", Salvatierra, GTO 38900, MEXICO, and Refugio Rodriguez-Vazquez, CINVESTAV-IPN, Avenida IPN 2508, Mexico City, Mexico.
Capacity of Trametes versicolor CDBB-H-1051 and native microflora soil to degrade a commercial pesticide was investigated under laboratory conditions. Fungus was grown in two different organic matrixes (stubble corn and sorghum straw) previous to add it to pesticide remediation system. A 23 factorial design was established. Eight experiments were developed by triplicate. Variables were pesticide concentration, organic matrix and ratio [organic matrix-fungus]/soil. Two levels for each variable were established: 500 and 5,000 ppm; stubble corn and sorghum straw; 5:95 and 10:90 ([organic matrix-fungus]/soil) respectively. Extensive degradation of the pesticide components in the bioaugmented system was achieved as shown on the chromatograms, after 130 days of treatment. Degradation of 1-bromide-2-nitrobencene, tetrachloro–m-xilene and decachlorobiphenyl was found (10.70±0.61 %; p < 0.001; α = 0.05) in the treatment with ratio 10:90, pesticide concentration 500 ppm and sorghum straw as organic matrix. The last two compounds are main organochlorine components of the commercial pesticide used. This degradation can be attributed to the presence of T. versicolor since no degradation was found on controls were fungus was not added. This research will allow establishing a methodology to reduce toxic compounds in the environment applied to local conditions.
Handout (.pdf format, 517.0 kb)