Monday, November 13, 2006
74-9
Global Gene Expression Analysis in Soybean in Response to the Causal Agent of Asian Soybean Rust (Phakopsora pachyrhizi Sydow).
Dilip Panthee1, Joshua Yuan1, David Wright2, James Marois3, Daniel Mailhot4, and Neal Stewart5. (1) University of Tennessee, 2431 Joe Johnson Drive, 2431 Joe Johnson Drive, Knoxville, TN 37996, United States of America, (2) University of Florida, 155 Research Road, 155 Research Road, Quincy, FL 32352, United States of America, (3) Univ. of Florida, 155 Research Rd., 155 Research Rd., Quincy, FL 32303, (4) North Florida Agriculture Research and Education Center, University of Florida, 155 Research Rd., Quincy, FL 32351, (5) Plant Sci Dept, Univ. of Tenn, "2431 Joe Johnson Dr,252 Plnt Sci Bd", "2431 Joe Johnson Dr,252 Plnt Sci Bd", Knoxville, TN 37996, United States of America
Asian soybean rust (ASR) caused by Phakopsora pachyrhizi Sydow is a potentially devastating disease posing a serious threat to the US soybean industry. Understanding plant host response to the pathogen at the molecular level is certainly important for control of the disease. The main objective of this study was to perform a global transcriptome profiling of P. pachyrhizi -exposed soybean using whole genome Affymetrix microarrays of soybean. Three week-old soybean cv. 5601T plants were inoculated with P. pachyrhizi and leaf samples were collected after 72 hours of inoculation. Twenty five genes were found to be differentially expressed, of which 16 were upregulated and 9 were down regulated. Most of the differentially expressed genes were defense and stress related, and only two were ASR specific. We picked five genes for real time RT-PCR to confirm the induction level, which confirmed the results from microarray analysis. To our knowledge, this is the first microarray analysis of soybean in response to ASR hence this research is expected to provide a basis for further studies including gene discovery and development of ASR-resistant soybean varieties.
Key words: Asian soybean rust (ASR), differential gene expression, gene profiling, Glycine max, microarray analysis, Phakopsora pachyrhizi
Key words: Asian soybean rust (ASR), differential gene expression, gene profiling, Glycine max, microarray analysis, Phakopsora pachyrhizi
Handout (.pdf format, 214.0 kb)