Louisiana is known for its abundance of water resources. Within Louisiana, approximately 69% and 58% of the river and lakes assessed, respectively, are adversely impacted by nonpoint source pollution (NPS). Agriculture production has been identified as one of the NPS pollution sources. In this two-year project, water samples from sugarcane run-off following major rainfall events was collected by automatic samplers placed at sugarcane production area on the natural levee of the Mississippi River, and its impact on water bodies within Barataria Basin were assessed. Suspended solids, nitrogen, phosphorus and pesticides in the water samples were analyzed. The results will be used to determine the total maximum daily load (TMDL) in this region, which will provide guidance for developing the best management practice to sugarcane production. The information will be incorporated into watershed planning, which will guide implementation of TMDLs within this part of the State.