Thursday, November 16, 2006 - 8:30 AM
313-2

Development of High Throughput Functional DNA Markers Based on Gene Families.

Jinfa Zhang1, Mingxiong Pang1, Chen Niu1, Wu Wang1, Fei Wang1, Danielle Miranda1, Jessica Curtiss1, Youlu Yuan2, and Shuxun Yu2. (1) NMSU Plant & Enivromental Sciences, Po Box 30003 Msc 3q, Las Cruces, NM 88003, United States of America, (2) China Cotton Research Institute, Anyang, China

Functional markers are molecular markers based on the polymorphisms present within genes involved in the expression of given traits. Many gene families including transcription factors have been identified to contain certain conserved motifs. The objective of this investigation was to develop high throughout functional marker systems targeting gene sequences based on AFLP analysis. Several marker systems including RGA-AFLP (for resistance gene analogs), TF-AFLP (for transcription factor genes), PPR-AFLP (for pentatricopeptide repeat protein genes), and PAAP (promoter-anchored amplified polymorphism) have been designed and successfully tested in cotton. Examples will be presented to demonstrate the applications of the new marker systems in search for candidate genes.  

Back to Gene Introgression and MarkerAssisted Breeding
Back to C01 Crop Breeding & Genetics

Back to The ASA-CSSA-SSSA International Annual Meetings (November 12-16, 2006)