Monday, November 13, 2006
59-16
Gene Pyramiding for Soybean Mosaic Virus Resistance Using Microsatellite Markers.
Ainong Shi1, Pengyin Chen1, Cuiming Zheng1, Anfu Hou1, and Shenlong Zhu2. (1) Univ of Arkansas, 115 Plant Science Bldg, Fayetteville, AR 72701, (2) Univ of Arkansas, 115 Plant Science Bldg, Fayetteville, AR 72701
Gene pyramiding is an important and effective approach to achieve multiple and durable resistance to various strains of Soybean Mosaic Virus (SMV) in soybean [Glycine max (L.) Merr.]. The objectives of this research were to pyramid Rsv1, Rsv3, and Rsv4 for SMV resistance with the aid of microsatellite markers in order to develop new soybean lines containing multiple resistance genes. A population of 84 lines derived from J05 (Rsv1, Rsv3) x V94-5152 (Rsv4) were developed, and six specific SSR markers were identified for SMV resistance genes. Two SSR markers Sat_154 and Satt510 were used for selecting lines having the Rsv1 gene, Satt560 and Satt726 for Rsv3, and Sat_254 and Satt542 for Rsv4. These SSR markers allowed for identification and selection of specific lines and individual plants containing different genes and for distinction of the homozygous and heterozygous lines or individual plants for all three resistance loci. Individual plants with homozygous alleles at three genetic loci (Rsv1Rsv1, Rsv3Rsv3 and Rsv4Rsv4) have been identified and new soybean germplasm is expected to be released with three genes combined for SMV resistance.