Wednesday, November 15, 2006
257-5

Identification of Hybrid Corn Seeds Using Near Infrared Reflectance Spectroscopy.

Tai-Gi Min1, Woo-Sik Kang1, and Suk Lee2. (1) Daegu Univ., College of Life and Environmental Science, 15 Naeri, Jinryang, Gyeongsan, 712-714, South Korea, (2) Yeungnam Univ, School of Biological Sciences, Gyongsan, 712-749, South Korea



For identification of F1 hybrid corn seeds a simple, fast, and non-destructing method to classify self-fertilized inbred from cross-fertilized F1 hybrid seeds was developed using near infrared spectroscopy (NIR). The hybrids (inbred lines) were 'Suwon19' (KS5 x KS6), 'Gwanganok' (Ga209 x DB544), and 'Suwonok' (KS7rhm x KS117) developed in Korea. The NIR reflectance spectra from 200 individual seeds were recorded in each hybrid and inbred seeds using a NIRSystem Model 5000 spectrometer from 1100 to 2500 nm with a resolution of 2 nm and analysed by a partial least squares 2 (PLS2) for calibration model. The calibration model computed by the PLS2 were applied to distinguish self-fertilized inbred and cross-fertilized hybrid seeds from the unknown 50 inbred or hybrid seeds. The cross-fertilized hybrid seeds of 'Suwonok' were classified from self-fertilized inbreeds of KS7rhm or KS117 with 92 to 98% accuracies and those of 'Suwon19' and 'Gwanganok' were classified with 100% accuracy using the PLS2 discriminant models. In conclusion, NIR spectroscopy could be used as a rapid and non-destructive method to test purity of hybrid corn seeds.