Monday, November 13, 2006
58-6
QTL Analysis for Yield and Other Agronomic Traits in Barley.
Bahman Yazdi-Samadi1, S.A. Peighambari1, Cyrus Mishani2, Gills Charmet3, and Ahmad Sarrafi4. (1) Univ of Tehran, Dept. of Agronomy, College of Agriculture, Daneshkadeh Ave., Karaj, Iran, (2) Dept. of Agronomy, College of Agriculture, Univ. of Tehran, Daneshkadeh Ave., Karaj, Iran, (3) INRA Clermont-Ferrand, 234 Ave. du Brezet, 63039, Clermont-Ferrand, Cedex, France, (4) Dept. of Biotechnology and Plant Breeding, BAP-IFR 40-ENSAT-INP, 18 Chemin de Bord Rouge, BP 32607-31326, Castanet, France
Seventy-two doubled haploid barley lines and their parents 'Steptoe' and 'Morex', were planted in a randomized complete block design with three replications at the research farm of the University of Tehran, Faculty of Agriculture, Karaj, Iran for two growing seasons. Ten agronomic traits including yield and yield components were studied. Analysis of variance showed significant differences between genotypes for most traits and for genotype-environment interactions (GxE). QTL analysis was carried out with MULTIQTL software using the GxE option. A genetic map comprising 327 molecular markers was recovered from 'Grain Genes' and used for QTL mapping. This map is fairly saturated with a total length of 1226 cM and an average marker spacing of 3.75 cM. QTLs were identified for all traits on all chromosomes except chromosome 6H. Highest LOD scores were obtained for the date of spike initiation and flowering on chromosome 2H (r2=31%) and for plant height (r2=34%) and spike length (r2=61%) on chromosome 3H. QTLs for 1000-seed weight (tsw), an indicator of drought tolerance, were found on chromosomes 1H (r2=21%) and 5H (r2=20%), with positive alleles from 'Morex', and on chromosome 7H with positive alleles from 'Steptoe'. Other QTLs for protein concentration (r2=26%) and seed yield (r2=21%) were found on chromosome 2H, with positive alleles for yield associated with alleles for late flowering.
Handout (.ppt format, 6219.0 kb)