Application of micronutrient foliar fertilizers in tank-mixtures with glyphosate may increase efficiency in glyphosate-resistant soybean production by reducing trips across the field. However, divalent and trivalent micronutrient cations may antagonize glyphosate control of weeds similarly to the hard water cations calcium (Ca) and iron (Fe). Water conditioners, such as diammonium sulfate, are commonly used to overcome the antagonistic effect of hard water cations. The objectives of this research were 1) to quantify the antagonism of glyphosate efficacy caused by various micronutrient formulations, and 2) to determine the extent that water conditioners may prevent the antagonism from occuring. Velvetleaf and giant foxtail were grown in 0.9 L pots in the greenhouse at 25±2șC with supplemental lighting. Treatments were applied when plants had 5 to 6 leaves using a single tip track sprayer. Treatment solutions were prepared in distilled water at a spray volume of 93 L/ha. Plants were evaluated for control 7 and 14 d after treatment. The water conditioner treatments were diammonium sulfate at 2% (w/w), NTANK at 0.5% and 1.0% (v/v), and CLASS ACT Next Generation at 2.5% (v/v). Micronutrients included in the study were Fe, manganese (Mn), copper (Cu), and zinc (Zn), each applied as sulfate, citric acid, EDTA, lignin sulfonate, and flavonal formulations. Two micronutrient blends were also evaluated. Both micronutrient blends eliminated all glyphosate activity, and the water conditioners did not reduce the antagonism significantly. Of the individual micronutrient formulations, those containing Fe caused the greatest antagonism of glyphosate, especially on velvetleaf. The 1.0% NTANK and 2% AMS treatments werethe most effective adjuvant treatments for overcoming micronutrient antagonism of glyphosate activity.