Limited research has been conducted on food-grade soybean [Glycine max (L.) Merr.] in the Mid-South. The objective of this study was to explore how row spacing, planting date, and irrigation affect agronomic and quality characteristics of food-grade soybean. Eight conventional, maturity group V cultivars ranging in seed size from 6 to 25 g per 100 seed were planted in two locations in northern Arkansas in 2002, 2003 and 2004. The small-seeded cultivars were V97-3000, Suzunishiki, MFS-591, and Camp. The large-seeded cultivars were MFL-552, V98-5089, Hutcheson, and SS-516. The cultivars were planted in a strip-plot design with three replications. Plant height, pubescence color, flower color, stand count, maturity, shatter, lodge, hilum color, yield, seed size, seed quality, percentage of greenseed, purple seed stain, mottling, calcium, protein, moisture, oil, raffinose, stachyose, sucrose, and seed hardness were collected for each plot. The plantings were conducted over 3 planting dates; late-April, mid-May, and early-June.