A soy-processing wastewater with high pH had extremely high amounts of Total Kjeldahl Nitrogen (TKN) and complex organics. Conventional wastewater treatment schemes were unable to degrade the TKN to concentrations required for discharging. An attached growth, packed-bed bioreactor (PBR) containing the white rot fungus (WRF), Phanerochaete chrysosporium, was evaluated for its effectiveness in treating the wastewater. Preliminary studies utilizing the PBR found that the high pH of the wastewater inhibited TKN and COD degradation. We hypothesize that adjustment of the wastewater pH before introduction into the bioreactor would overcome this inhibition and result in a greater reduction of the TKN and COD. Specifically, the first objective was to investigate the effectiveness of pH adjustment of the wastewater before treatment in the bioreactor. The second objective was to investigate the effectiveness of treating the wastewater with the enzyme culture solution obtained from the reactor. Continuous adjustment of pH during recycling of the wastewater within the PBR resulted in an increase in the amount of TKN and COD degraded. Over 90% of the TKN and approximately 33% of the COD was removed during treatment within the PBR. Treatment of the wastewater with the fungal enzyme solution had little effect on TKN concentration. However, there was a 50 to 67% decrease in the total amount of COD found in the wastewater. The pH in the treatment flasks remained constant. With the improved pH control in the PBR during recycling events, a greater amount of TKN and COD were removed from the wastewater. Treatment of the wastewater with fungal enzymes resulted in a significant decrease in COD concentration. A combination of treatments, inside and outside the PBR, could lead to complete removal of both COD and TKN thus allowing for discharge of the treated wastewater into surface water streams.