Many of the linkage maps that have been assembled in cotton are based on interspecific crosses (Gossypium hirsutum L. X Gossypium barbadense L. ). The primary reason for this is the low rate of polymorphism usually found in intraspecific crosses of upland cotton (Gossypium hirsutum L.). As more transportable SSR markers become available, the development of intraspecific linkage maps will occur. We constructed an intraspecific linkage using 188 recombinant inbred lines (RIL) that were developed by single hill (bulked progeny row) procedure from 94 F₂ plants from the cross of HS 46 by MARCABUCAG8US1-88. The two parents were genotyped with 943 Simple Sequence Repeat (SSR) markers (BNL, CIR, JESPR, and CM). The 211 markers (22%) that were polymorphic between the parents were used to genotype the 188 RIL. In addition, these SSR markers were assigned to chromosomes using aneuploid lines. The resulting intraspecific linkage map should be a valuable resource for geneticists and breeders.