The Lolium and Agrostis genera include the major cool season turfgrass species such as perennial ryegrass (L. perenne) and creeping bentgrass (A. stolonifera). These species are self-incompatible, outcrossing, and perennial. Perennial ryegrass is diploid (2n=2x=14) whereas creeping bentgrass is allotetraploid (2n=4x=28). We have previously constructed linkage maps of each species and also studied their genome relationships through a comparative mapping by a common set of cereal RFLP markers. No evidence of large-scale chromosomal rearrangements between the species was detected despite the fact that they belong to different tribes. However, the number of common markers mapped on both linkage maps was limited. In this recent study, EST-RFLP markers derived from creeping bentgrass, which were previously mapped on the creeping bentgrass, were screened and mapped on ryegrass. In addition, a set of cereal EST-SSR primers has been selected and tested to investigate if they could be employed on both ryegrass and creeping bentgrass mapping. Results of the genome relationship between the two species and applications of the cereal EST-SSR markers for a comparative mapping in the Poaceae family will be discussed.