Our current study shows that the feminizing effect of Tilletia buchloeana Kell. & Swing. infection on the sex expression of dioecious buffalograss (Bouteloua dactyloides (Englm.) Columbus; syn. Buchloë dactyloides (Nutt.) Englm.) causes a shift in the sex ratios from approximately 1:1 to almost 100% female sex form in the population. Our initial observations also suggest that the overall effect of T.buchloeana infection on the sexuality and development of buffalograss unisex florets mimics tasselseed mutants in maize. Fluorescent microscopic images of the florets destined to be male verified that Ts-2 gene indeed causes the loss of nuclei as a result of programmed cell death in the sub-epidermal cells of degenerating gynoecium. However, the fungus supersedes the expression of Ts-2 gene upon infection, thereby, inducing the development of pistils in an otherwise staminate floret, a condition analogous to maize ts-2 mutants. A portion of Ts-2 homologue was amplified out of buffalograss genome using SSR primers designed from maize Ts-2 gene sequence information followed by 3' and 5' RACE to get the full length Ts-2 gene from buffalograss. In-situ hybridization was performed which indicated the spatial expression of the gene only in the sub-epidermal layer of the gynoecium, as expected. Real time RT-PCR analysis over different stages of plant development enabled us to quantitatively estimate 5-8 fold reduction of Ts-2 expression in infected vs. non-infected male buffalograss. Detailed morphological analysis supplemented with scanning electron microscopy revealed other secondary effects of fungal infection like induction of additional pistillate floret per spikelet in each sex form besides early and increased flowering leading to ten to 15-fold increase in ovary production. The infection by the fungus reallocates plants resources from being utilized for vegetative growth towards a dramatic increase in ovary production thereby enhancing the potential for overall seed yield.