Reclaimed soils following strip mining can be a source of mineral and organic colloids which may mediate heavy metal transport to groundwater. Colloid generation and associated metal migration in these sites may be accentuated by disturbance of soil and geological strata as well as biosolid amendment applications. Two sites were chosen for this study, one in the Appalachian region of southwest Virginia and one in the eastern Kentucky coal fields, to observe colloid transport under simulated rainfall conditions. Intact reclaimed soil monoliths were retrieved from sandstone derived soils in southwestern Virginia. Reclaimed monoliths from eastern Kentucky were recreated in the lab. Intact undisturbed soil monoliths representing the soils before mining were also sampled for comparison. Leaching experiments with de-ionized water at a rate of 1.0 cm/h involved five cycles of 6 hours each, with intermittent surface drying between cycles via an electric fan. First cycle eluents of a disturbed Virginia soil monolith had an average colloid concentration of 910 mg/L, which was gradually reduced to 0 mg/L in the last cycle. Elution pH remained near 6.0 for the duration of the cycles, while EC gradually decreased from 910 uS/cm to 50 uS/cm with each subsequent cycle. In contrast, colloid concentrations in eluents of an undisturbed Virginia soil monolith averaged 175 mg/L in the first cycle and declined to 0 mg/L by the final cycle. Elution EC was initially 423 uS/cm and decreased to 261 uS/cm by the final cycle. The results suggest significant increases in colloid generation following the disturbance that could induce heavy metal transport and further groundwater quality deterioration.