Allelopathic response of plants to environmental changes largely depends on the plant genotype. In our study we tried to analyze the response of different sugar beet varieties to several growth-stimulating hormones applied in vitro. The processes of morphogenesis were investigated in sugar beet tissue culture in vitro using 14 Ukrainian and foreign cultivars. Explants (petioles, basal tissue, hypocotyls and leaf plates) were placed on MS culture medium supplemented with BAP 1 mg/L and cultured under intensive illumination. Shoot formation was observed 2-4 days later. In most cases the plant regeneration went trough formation of buds on the surface of explants. The regeneration rate (the number of explants produced shoots/total number of regenerants) varied significantly among the varieties and explants: for petioles, for example, it ranged from 25% for Ramonskaya MS-54 to 40% for Uladovskaya MS-5. Some varieties showed very high response rate from certain type of explants only: for example, Mejotnensky hybrid-18 produced regenerants mostly from basal tissue. The strongest response rate to externally applied hormones across all explants was found in Ramonskaya monoseed-47 variety. Therefore the allelopathic response of plants to externally applied hormones is highly genotype-dependent.