The leaf rust resistance gene Lr52, which is derived from an Iranian landrace, was recently mapped to chromosome 5BS. An independent study also mapped a leaf rust resistance gene from Iranian landrace PI 289824 to the same chromosomal region. These were the first reports of leaf rust resistance mapping to chromosome 5BS. In order to determine the prevalence of these gene(s) in Iranian landraces, one hundred Iranian landraces were obtained and screened for reaction to leaf rust. Resistant accessions were then tested with DNA markers previously reported to be linked to the gene(s) on chromosome 5BS. The prevalence of resistance due to these genes is discussed along with the implications for the use of Iranian landraces to improve leaf rust resistance of modern cultivars.