Bermudagrass (Cynodon dactylon) is an important warm-season grass used for pasture, turf, and soil stabilization in sub-temperate and tropical regions of the world. Genetic engineering efforts to improve bermudagrass tolerance to stresses such as temperature extreme, drought, diseases, and insect pests require plant regeneration through tissue culture. In order to optimize tissue culture technique for regenerating bermudagrass, culture response of ‘Brazos' and ‘PRC 58' was investigated. Pieces of young inflorescence tissue were cultured on solid MS nutrient medium supplemented with 2,4-D, Dicamba, or Picloram at 0,1,3,5, and 7 mg L-1 concentrations. Calli were initiated from explant tissue except at 0 mg L-1 auxin, and higher initiation frequencies occurred at levels higher than 1 mg L-1. After a few subcultures, some calli developed compact sectors characteristic of regenerable calli. 2,4-D was superior to dicamba or picloram in inducing callus growth. Brazos responded similarly in callus initiation to 2,4-D concentrations of 3,5, and 7 mg L-1 while PRC 58 responded best to 5 and 7 mg L-1 concentrations. Several plantlets of PRC 58 were successfully obtained from calli grown with 3 mg L-1 2,4-D, while most calli of Brazos suffered severe contamination prior to transfer to regeneration medium. Plant regeneration was demonstrated for PRC 58. Culture protocol for different bermudagrass cultivars will require optimization because of potential differential cultivar response to specific culture protocols.