Recombinant inbred line (RIL) populations developed through continuous selfing from the cross between two contrasting parental inbreds can be used to estimate heritabilities and to map quantitative trait loci (QTL) of relevant traits in maize. Our objective is to characterize a RIL population derived from the cross between inbreds CM L161 and B73o2. Inbred CM L161 is a tropical quality protein maize (QPM) line released by CIMMYT with flint endosperm and wide cobs. B73o2 is a floury B73 carrying a non-functional mutation in the O2 gene, which regulates the expression of alpha-zeins. B73o2 flowers earlier than CML161 and is more susceptible to pests and disease. A total of 300 S6 RILs were developed by selfing the F1 hybrid and subsequent generations. These RILs segregate for several traits such as maturity, grain attributes, grain yield, test weights, plant height, plant color, tassel size, and response to diseases including aflatoxin accumulation. The RIL population and the parental inbreds were evaluated in replicated trials in two Texas locations, Weslaco and College Station under inoculation with Aspergillus flavus. Significant differences were detected for maturity measured as days from planting to flowering, grain yield, and grain texture. The phenotypic data collected from these two locations will be used to estimate heritabilities and genetic correlations for relevant traits and combined with molecular marker data to map QTLs for aflatoxin and associated traits.